CRISPR CAS-9 Gene Editing Technology Course - Bio Help Learning

CRISPR CAS-9 Gene Editing Technology Course

This course provides a comprehensive understanding of CRISPR CAS-9 Gene Editing Technology Course and equips students with the necessary skills to analyze CRISPR CAS-9 experiments. It combines theoretical knowledge with practical applications, preparing students to contribute to the rapidly evolving field of genomics and Data analysis

About Course

This course provides a comprehensive understanding of CRISPR CAS-9 Gene Editing Technology Course and equips students with the necessary skills to analyze CRISPR experiments. It combines theoretical knowledge with practical applications, preparing students to contribute to the rapidly evolving field of genomics and Data analysis

CRISPR CAS-9 Gene Editing Technology Course

Day-1

Introduction to CRISPR-Cas9 Technology-1

Welcome and Course Overview
Introduction to Genome Editing
Historical Development of CRISPR-Cas9
Basics of Molecular Biology
Overview of DNA, RNA, and Proteins
Central Dogma of Molecular Biology

Day-2

Introduction to CRISPR-Cas9 Technology-2

Introduction to CRISPR Biology
Different CRISPR Systems
Cas9 Structure and Function
Mechanism of CRISPR-Cas9
PAM Sequences and Target Recognition
Types of Genetic Modifications

Day-3

Designing CRISPR Experiments

Choosing Target Genes
Off-Target Effects and Mitigation Strategies
Designing Guide RNA (gRNA)
Tools for gRNA Design
Hands-on Exercise: gRNA Design

Day-4

Designing CRISPR Experiments-02

Selection and Validation of CRISPR-Edited Cells
Detection Techniques: PCR, Sequencing, and more
Analysis of Off-Target Effects
Hands-on Exercise: Analyzing CRISPR-Edited Cells

Day-5

Designing CRISPR Experiments-03

Principles of read alignment
Overview of popular alignment tools (e.g., STAR, HISAT2)
Alignment quality control and metrics

Day-6

Hands-on Alignment Session

Practical session on aligning reads to the reference genome
Troubleshooting alignment issues
Visualization of alignment results

Day-7

Gene Expression Quantification

Methods for quantifying gene expression
Feature counting and expression estimation
Normalization techniques

Day-8

Differential Expression Analysis Basics

Introduction to differential expression analysis
Statistical concepts and hypothesis testing
Overview of common tools (DESeq2, edgeR)

Day-9

Differential Expression Analysis Practical

Hands-on session on performing differential expression analysis
Interpretation of results and fold-change analysis
Visualization of differentially expressed genes

Day-10

Functional Enrichment Analysis

Introduction to functional annotation
Enrichment analysis methods (GO, KEGG)
Visualization of enrichment results

Day-11

Alternative Splicing Analysis

Concepts of alternative splicing
Tools for detecting and quantifying splice variants
Interpretation of alternative splicing events

Day-12

Integrative Analysis with Other Omics Data

Integration of RNA-Seq with proteomics and metabolomics data
Practical sessions on multi-omics integration
Systems biology approaches

Day-13

Advanced Topics in RNA-Seq

Single-cell RNA-Seq analysis
Long-read sequencing applications
Emerging technologies and trends

Day-14

Project Presentation and Future Directions

Introduction to functional annotation
Enrichment analysis methods (GO, KEGG)
Visualization of enrichment results

Day-15

Functional Enrichment Analysis

Participants present their RNA-Seq projects
Feedback and discussion
Future trends in RNA-Seq and genomics research

Educator - Not assign

update soon

RNA SEQ DATA ANALYSIS COURSE

Mode - Online

Certificate Provided

Hours 10 Hours

Time 7:00 to 8:00 PM IST

Last Date available soon

Course Start Date available Soon

Recorded lecture Provided after session

Certificate

FAQ

How do I join the session?

Session Joining link will be send to your register email on the day of session start you can join session by clicking joining link.

What if I missed live sessions? Will this be recorded?

Yes! All of our Live sessions are recorded and you can access recorded session after live classes, recorded session link will be send to your respective email after live classes. recorded session will be available for 30 days after live classes

What is timing of sessions?

All Session are evening sessions time of session is between 7:00 to 8:00 PM IST.

What about the refund of the course?

Please read refund policy in refund policy section page.

What Prerequisites knowledge required to join the course?

No, any short Prerequisites knowledge you required to join the course.