CRISPR CAS-9 Gene Editing Technology Course
This course provides a comprehensive understanding of CRISPR CAS-9 Gene Editing Technology Course and equips students with the necessary skills to analyze CRISPR CAS-9 experiments. It combines theoretical knowledge with practical applications, preparing students to contribute to the rapidly evolving field of genomics and Data analysis
Educator
announce soon
Difficulty
Intermediate
Mode
Live
Language
English
About Course
This course provides a comprehensive understanding of CRISPR CAS-9 Gene Editing Technology Course and equips students with the necessary skills to analyze CRISPR experiments. It combines theoretical knowledge with practical applications, preparing students to contribute to the rapidly evolving field of genomics and Data analysis
CRISPR CAS-9 Gene Editing Technology Course
Day-1
Introduction to CRISPR-Cas9 Technology-1
- Welcome and Course Overview
- Introduction to Genome Editing
- Historical Development of CRISPR-Cas9
- Basics of Molecular Biology
- Overview of DNA, RNA, and Proteins
- Central Dogma of Molecular Biology
Day-2
Introduction to CRISPR-Cas9 Technology-2
- Introduction to CRISPR Biology
- Different CRISPR Systems
- Cas9 Structure and Function
- Mechanism of CRISPR-Cas9
- PAM Sequences and Target Recognition
- Types of Genetic Modifications
Day-3
Designing CRISPR Experiments
- Choosing Target Genes
- Off-Target Effects and Mitigation Strategies
- Designing Guide RNA (gRNA)
- Tools for gRNA Design
- Hands-on Exercise: gRNA Design
Day-4
Designing CRISPR Experiments-02
- Selection and Validation of CRISPR-Edited Cells
- Detection Techniques: PCR, Sequencing, and more
- Analysis of Off-Target Effects
- Hands-on Exercise: Analyzing CRISPR-Edited Cells
Day-5
Designing CRISPR Experiments-03
- Principles of read alignment
- Overview of popular alignment tools (e.g., STAR, HISAT2)
- Alignment quality control and metrics
Day-6
Hands-on Alignment Session
- Practical session on aligning reads to the reference genome
- Troubleshooting alignment issues
- Visualization of alignment results
Day-7
Gene Expression Quantification
- Methods for quantifying gene expression
- Feature counting and expression estimation
- Normalization techniques
Day-8
Differential Expression Analysis Basics
- Introduction to differential expression analysis
- Statistical concepts and hypothesis testing
- Overview of common tools (DESeq2, edgeR)
Day-9
Differential Expression Analysis Practical
- Hands-on session on performing differential expression analysis
- Interpretation of results and fold-change analysis
- Visualization of differentially expressed genes
Day-10
Functional Enrichment Analysis
- Introduction to functional annotation
- Enrichment analysis methods (GO, KEGG)
- Visualization of enrichment results
Day-11
Alternative Splicing Analysis
- Concepts of alternative splicing
- Tools for detecting and quantifying splice variants
- Interpretation of alternative splicing events
Day-12
Integrative Analysis with Other Omics Data
- Integration of RNA-Seq with proteomics and metabolomics data
- Practical sessions on multi-omics integration
- Systems biology approaches
Day-13
Advanced Topics in RNA-Seq
- Single-cell RNA-Seq analysis
- Long-read sequencing applications
- Emerging technologies and trends
Day-14
Project Presentation and Future Directions
- Introduction to functional annotation
- Enrichment analysis methods (GO, KEGG)
- Visualization of enrichment results
Day-15
Functional Enrichment Analysis
- Participants present their RNA-Seq projects
- Feedback and discussion
- Future trends in RNA-Seq and genomics research
Educator – Not assign
update soon
RNA SEQ DATA ANALYSIS COURSE
Mode – Online
Certificate Provided
Hours 10 Hours
Time 7:00 to 8:00 PM IST
Last Date available soon
Course Start Date available Soon
Recorded lecture Provided after session
Certificate
FAQ
Session Joining link will be send to your register email on the day of session start you can join session by clicking joining link.
What if I missed live sessions? Will this be recorded?
Yes! All of our Live sessions are recorded and you can access recorded session after live classes, recorded session link will be send to your respective email after live classes. recorded session will be available for 30 days after live classes
All Session are evening sessions time of session is between 7:00 to 8:00 PM IST.
What about the refund of the course?
Please read refund policy in refund policy section page.
What Prerequisites knowledge required to join the course?
No, any short Prerequisites knowledge you required to join the course.