RNA SEQ Data Analysis

This course provides a comprehensive understanding of RNA SEQ DATA ANALYSIS and equips students with the necessary skills to  analyze RNA SEQ experiments. It combines theoretical knowledge with practical applications, preparing students to contribute to the rapidly evolving field of genomics and Data analysis

Registration

Difficulty

Intermediate

Mode

Live

language

Language

English

Payment
Paypal

About Course

This course provides a comprehensive understanding of RNA SEQ DATA ANALYSIS and equips students with the necessary skills to  analyze RNA SEQ experiments. It combines theoretical knowledge with practical applications, preparing students to contribute to the rapidly evolving field of genomics and Data analysis

RNA SEQ Data Analysis

Day 01

Introduction to Sequencing Technologies

  • Emulsion PCR/ Bridge PCR
  • Maxel-Geibert sequencing
  • Sanger Sequencing
  • SOLID Sequencing
  • SMRT
  • Nanopore Sequencing
  • Ion torrent sequencing
  • Whole Genome Shortgun Sequencing
  • Pyrosequncing, etc

Day 02

Linux

  • Introduction to Linux
  • Installation of Linux
  • Package manager in Linux
  • Basics and advance command line in Linux
  • File Handling and File manupulation in Shell
  • Statics and experimental Design of RNA seq

Day 03

R-Programming and R studio

  • Installation of R and R studio
  • Data types and R studio functionality
  • Data type/ Data structure
  • Vectors and functions
  • different types functions
  • Fetching

Day 04

R Programming

  • Sequence Function 
  • Print Function
  • Manupulation of Vectors
  • replacing of vectors
  • R programming libraries
  • Bioconductor Packages etc.

Day 05

Bioinformatics Databases and Tools

  • Different types of Biological Databases.
  • NCBI, Ensembl, PDB, plant TF, chembl, Pubchem
  • Primer designing 
  • Restriction mapping

Day 06

NGS workflow

  • workflow of NGS RNA seq
  • Files and file formats
  • Single end paired end Data analysis
  • experimental design of RNA seq
  • Data Downloading using various approches.

Day 07

Quality control of RNA seq Data

  • FASTQ file format understanding
  • Quality check of Data
  • FastQC, MultiQC
  • Trimming of Data using various trimming tools

Day 08

Alignment to reference Genomes

  • Reference genome alignment.
  •  Indexing of genome
  • Different alignment tools
  • HISAT2/Kallisto/STAR
  • BAM SAM File formats
  • sorting and Indexing

Day 09

Genome Annotation

  • Genome annotation
  • Integrative Genomics Viwer (IGV)
  • Chromosome position and gene analysis
  • HTseq Count and FPKM and TPM counts

Day 10

Differential expressed Genes

  • PCA plot
  • BCA plot
  • QRM plot
  • Volcano plot
  • Upregulated and regulated genes analysis

Day 11

Gene Cluster analysis

  • Heatmaps and clustering
  • Heatmap visualization and interpretation.
  • Phetmap and complex heatmap generations

Day 12

Gene Ontology analysis

  • Gene Ontology analysis using R programming
  • Interpretation of Gene ontology
  • Various graph Generation of GO

Day 13

Visualization of GO

  • Gene ontology analysis using DAVID
  • Bar plot and dotplot generation of Gene ontology data
  • ggplot, venn digram, etc

Day 14

KEGG Analysis

  • Enrichment analysis methods (KEGG)
  • Using R and Web based tool

Day 15

Report Writting

  • Report writting and presentation of data
  • Doubt Solving Session

Reviews

RNA SEQ DATA ANALYSIS COURSE

Mode - Online

Certificate Provided

Hours 15-20 Hours

Time 7:00 to 8:00 PM IST

Last Date 13 April 2025

Course Start 15 April 2025

Recorded lecture Provided after session

Certificate

FAQ

Session Joining link will be send to your register email on the day of session start you can join session by clicking joining link.

Yes! All of our Live sessions are recorded and you can access recorded session after live classes, recorded session link will be send to your respective email after live classes. recorded session will be available for 30 days after live classes

All Session are evening sessions time of session is between 7:00 to 8:00 PM IST.

Please read refund policy in refund policy section page.

No, any short Prerequisites knowledge you required to join the course.

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