Bioinformatics Workshop Module 14 February 2023

Practical Module 1 : Genomics

Introduction to Bioinformatics and computational biology
Introduction History Applications and Areas of Bioinformatics and Computational Biology
Introduction to Various biological databases
Genomic DNA / Gene/ Protein Sequence retrieval from various biological databases
Gene sequence analysis
Molecular weight analysis of gene
GC% , AT%, Nucleotide composition,
Restriction Enzymes analysis,
Primer Designing, Probe Designing,
cDNA Conversion, Introns and exons analysis,
SNP analysis, EST analysis,
SSR analysis, STS analysis, Promoter analysis, Transcription start site analysis,
UTR analysis, Fuzzy techniques,
Sequence chopping analysis,
Pairwise alignment,
Multiple sequence alignment,
local alignment, global alignment,
Dynamic Programming
Conserved region analysis, Phylogenetic analysis

Protein sequence analysis
Protein amino acid composition
Molecular weight analysis,
Iso electric point
Biochemical studies such as hydrophobic , hydrophilic, polar, non-polar, positive residues, negative
residues, Aromatic analysis,
Protein Primary structure prediction,
Protein secondary structure prediction
Ramachandran Plot mapping
Loop modeling
Side chain modeling
Protein 3D Structure Analysis
Protein 3D structure retrieval and analysis
Protein 3D structure mutagenesis

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