BIO HELP LEARNING COURSE

Introduction to NGS and Sequencing QC

Understand next-generation sequencing from library to FASTQ, and learn to assess and clean sequencing data with FastQC, MultiQC and fastp.

Genomics

◉ Beginner◷ 10 Hours▣ Certificate Included

Course Information

Everything you need to know before enrolling.

MODE OF LEARNINGOnline

LANGUAGEEnglish

Pre-Register for Live Training

Registration for the next live batch opens soon. Pre-register and our team will contact you with the batch dates, fees and registration link as soon as it opens.

Live online classes
Group & college batches
Certificate on completion

Pre-register for live training → Ask on WhatsApp Or email [email protected]

▤ Overview ☷ Curriculum ♙ Instructor ☆ Reviews ▣ FAQs

▤

About This Course

Every NGS analysis, from RNA-seq to variant calling, starts with understanding the raw data. This course explains how sequencing works and how to judge whether your data is good enough to analyse.

You learn sequencing technologies and library preparation, read FASTQ files and Phred quality scores, run FastQC and MultiQC, trim adapters and low-quality bases with fastp, and write a clear QC report.

Who this course is for

Students starting any NGS-based project
Researchers who receive sequencing data from a core facility
Learners preparing for RNA-seq, WGS, WES or epigenomics courses

Prerequisites

Basic molecular biology and the Linux command line (or our Linux course).

Final project

Run quality control on a small public dataset before and after trimming and write a QC report with clear recommendations.

◎

What You’ll Learn

✓Explain how major sequencing platforms work

✓Describe library preparation and sequencing design

✓Read FASTQ files and Phred quality scores

✓Run and interpret FastQC and MultiQC reports

✓Trim adapters and low-quality reads with fastp

✓Download public data from SRA and ENA

✓Write a QC report and decide if data is analysis-ready

⌘

Tools & Technologies

IlluminaSRA ToolkitENAFastQCMultiQCfastpTrimmomaticLinuxConda

Course Curriculum

What you will learn, module by module

8 modules 10 hours of training 32 topics Certificate on completion

- From Sanger to NGS
- Sequencing by synthesis
- Short-read vs. long-read platforms
- Choosing a technology for a study
- Fragmentation, adapters and indexes
- Single-end vs. paired-end reads
- Read length and sequencing depth
- Replicates and controls
- RNA-seq, WGS and WES
- ChIP-seq and ATAC-seq
- Metagenomics and amplicon sequencing
- Single-cell sequencing
- FASTQ structure line by line
- Phred scores, Q20 and Q30
- Paired files R1 and R2
- Common file naming conventions
- Finding datasets in SRA, ENA and GEO
- Reading study metadata
- Downloading with the SRA Toolkit
- Checking downloaded files
- Running FastQC on many samples
- Reading each FastQC module
- Combining reports with MultiQC
- Spotting adapter, duplication and GC problems
- Adapter trimming with fastp
- Quality and length filtering
- Comparing before and after QC
- When not to trim
- Writing a QC summary
- Deciding if data is analysis-ready
- What comes after QC in each workflow
- Mini project: QC report on a public dataset

♙

Your Instructor

Bio Help Learning Educator

Bioinformatics Educator & Mentor

Learn from experienced educators and practitioners through practical, career-focused bioinformatics training.

Frequently Asked Questions

6 answers about this course

Do I need Linux knowledge?

Basic commands help. If you are new to Linux, take our Linux and Command Line course first or alongside.

Does this course include alignment or variant calling?

No. It focuses on sequencing concepts and quality control, which prepare you for our RNA-seq, WGS, WES and epigenomics courses.

Which course should I take next?

RNA-Seq Data Analysis, WGS Analysis or Metagenomics, depending on your research area.

Will I get a certificate?

Yes, after completing all modules, the quizzes and the final project.

How do I get fee and batch details?

Pre-register on this page or contact us on WhatsApp, and our team will share the details.

How long will I have access to the course?

You get full access to all course materials for 3 months from the date of enrollment.

Still have a question?Our team usually replies within one working day.

Ask on WhatsApp →

WHAT OUR LEARNERS SAY

Real Stories. Real Growth.

Hear from learners building practical skills with Bio Help Learning.

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I really enjoyed the course and found it very useful: it gave a clear and practical overview of the whole WGS workflow, from raw reads to a VCF, and the hands-on sessions helped a lot.

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As i am from biotechnology background this workshop, actually very helpfull for me

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Learn about different molecular docking tools.

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From this workshop I learned many new things such as docking, about new websites,how to use Swiss software,basic bioinformatics etc, it's very helpful for me.

Sayan DuttaUniversity of CalcuttaBioinformatics Training

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The workshop was informative and provided valuable insights into bioinformatics tools and techniques.

Santhosh M EReva University BangaloreBioinformatics Tools and Techniques

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The Drug Discovery Workshop was highly informative and helped me gain valuable knowledge about the drug discovery process, including molecular docking, drug–target interactions, and computational tools used in drug design.

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