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What You’ll Learn
✓Understand the principles and applications of Whole Exome Sequencing
✓Perform quality assessment of WES sequencing data
✓Perform read trimming and preprocessing
✓Align sequencing reads to a reference genome
✓Process and assess aligned BAM files
✓Perform SNP and Indel variant calling
✓Filter and prioritize genomic variants
✓Annotate variants using established databases
✓Interpret variants based on genomic and functional information
✓Generate a complete WES analysis report
Tools & Technologies
GATKBWA-MEM2FastQCSamtoolsBCFtoolsVEPIGV
Course Curriculum
Introduction to Whole Exome Sequencing & WES Workflow · 60 mins — practical lessons, demonstrations and guided work included in this module.
WES Data Structure, FASTQ Files & Quality Assessment · 60 mins — practical lessons, demonstrations and guided work included in this module.
Quality Control & Read Preprocessing · 60 mins — practical lessons, demonstrations and guided work included in this module.
Reference Genome Preparation & Read Alignment · 60 mins — practical lessons, demonstrations and guided work included in this module.
SAM/BAM Processing & Alignment Quality Assessment · 60 mins — practical lessons, demonstrations and guided work included in this module.
Duplicate Marking, Base Quality & GATK Preprocessing · 60 mins — practical lessons, demonstrations and guided work included in this module.
Variant Calling for SNPs & Indels · 60 mins — practical lessons, demonstrations and guided work included in this module.
Variant Quality Control & Variant Filtering · 60 mins — practical lessons, demonstrations and guided work included in this module.
Variant Annotation Using VEP · 60 mins — practical lessons, demonstrations and guided work included in this module.
Variant Prioritization & Functional Interpretation · 60 mins — practical lessons, demonstrations and guided work included in this module.
Clinically Relevant Variant Analysis & Database Interpretation · 60 mins — practical lessons, demonstrations and guided work included in this module.
Variant Visualization Using IGV · 60 mins — practical lessons, demonstrations and guided work included in this module.
End-to-End WES Analysis on Real Dataset · 60 mins — practical lessons, demonstrations and guided work included in this module.
WES Results Visualization & Report Generation · 60 mins — practical lessons, demonstrations and guided work included in this module.
Mini Project: Complete WES Data Analysis Workflow · 60 mins — practical lessons, demonstrations and guided work included in this module.
Your Instructor
Bio Help Learning Educator
Educator | MentorLearn from experienced educators and practitioners through practical, career-focused bioinformatics training.
Student Reviews
AV
“Excellent course! The content is practical and the projects made the concepts much easier to apply.”
Ananya VermaPhD Scholar, India★★★★★RM
“Hands-on projects made all the difference. Highly recommended for anyone interested in NGS.”
Rahul MehtaResearch Associate, Germany★★★★★SM
“Very detailed and industry-relevant content. I feel much more confident now.”
Sneha PatelBioinformatics Analyst, India★★★★★Frequently Asked Questions
Need help? →What is Whole Exome Sequencing?⌄
Whole Exome Sequencing is a sequencing approach that focuses primarily on protein-coding regions of the genome, known as exons.
Who can join this WES training?⌄
The training is suitable for biotechnology, bioinformatics, life science, genetics, genomics and related students, researchers and professionals.
Do I need prior WES experience?⌄
No. The course starts with WES fundamentals and progressively moves toward practical data analysis.
Will this course include hands-on analysis?⌄
Yes. The curriculum is designed around practical WES data analysis workflows and real-world datasets.
Which tools will I learn?⌄
You will work with tools including GATK, BWA-MEM2, FastQC, Samtools, BCFtools, VEP and IGV.
Will I learn variant calling?⌄
Yes. The course covers SNP and Indel variant calling, quality control and filtering.
Will I learn variant annotation?⌄
Yes. Variant annotation and functional interpretation are covered using VEP and relevant genomic resources.
Will I receive a certificate?⌄
Yes, participants can receive a certificate upon successful completion of the training.
Will I get course materials?⌄
Yes. Participants can receive supporting learning materials and practical resources as provided with the training program.
Is there a project at the end of the course?⌄
Yes. Day 15 is dedicated to a mini project involving a complete WES analysis workflow.
